• 介紹： N-(2-羥乙基)哌嗪-N’-(2-乙磺酸)，是可滿足于所有生物學(xué)研究的最佳緩沖液之一。絕大部分生理PHS下HEPES是一種兩性離子有機(jī)化學(xué)緩沖劑，屬于Good’s緩沖試劑之一，pH 6.0-8.5范圍內(nèi)的緩沖能力最強(qiáng)。HEPES應(yīng)用非常廣泛，包括：1）用作細(xì)胞培養(yǎng)的緩沖體系，常用工作濃度為10-25 mM；與無(wú)機(jī)碳酸氫鹽緩沖系統(tǒng)相比，在組織或者器官培養(yǎng)中表現(xiàn)出更好的pH控制能力。不像碳酸氫鹽，HEPES不為細(xì)胞供給營(yíng)養(yǎng)。只有當(dāng)需要長(zhǎng)時(shí)間在CO2培養(yǎng)箱內(nèi)處理細(xì)胞，才會(huì)加入培養(yǎng)基內(nèi)以增強(qiáng)緩沖能力。2）用作兩性電解質(zhì)溶液；HEPES能溶解兩性電解質(zhì)，制備＜1 pH單位寬度的pH梯度以用于等電聚焦電泳實(shí)驗(yàn)；3）用作電鏡觀察蛋白沉積技術(shù)合適的緩沖體系，因HEPES不會(huì)對(duì)金屬基板造成負(fù)面影響；4）可用作定量和選擇性測(cè)定抗原-抗體反應(yīng)的緩沖試劑。

• 熔點(diǎn)： 234-238℃

• 外觀： 白色結(jié)晶粉末

• 溶解性： 溶于水(2.25mol/L,0℃)

• 儲(chǔ)存條件： RT

• 用途： 本品僅供科研，不得用于其它用途。

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

• 43. [2.6] Tan Lin et al."Monochasma Savatieri Aqueous Extract inhibits Human Breast Cancer Cell Line Migration and Adhesion Without Generating Toxicity."Anti-Cancer Agents in Medicinal Chemistry.2024 Aug;24(13):982-989

• 42. [IF=6.1] Li Wang et al."Peptidomics-based Study of Antihypertensive Activity: Discovery of Novel ACE Inhibiting Peptides from Peanut Yogurt."Food & Function".2024 May;:

• 41. [IF=5.2] Yuhan Yan et al."Browning mechanism of sorbitol-glycine system in the presence of glycerol: Regulating conversion of sorbitol to reducing sugars and water status."Food Bioscience".2024 Jun;59:103901

• 40. [IF=9.1] Jian Wang et al."Regulation of uniformity and electric field distribution achieved highly energy storage performance in PVDF-based nanocomposites via continuous gradient structure."CHINESE CHEMICAL LETTERS".2024 Mar;:109714

• 39. [IF=8.2] Shuwei Luo et al."Solubility-permeability interplay of a supersaturated lutein delivery system constructed by glycosylated stevioside and hydroxypropyl-methylcellulose."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.2024 Feb;258:128791

• 38. [IF=5.2] Zemin Ji et al."Synthesis of ZIF-90 based nanozyme with excellent carbonic anhydrase activity."COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS.10.1016/j.colsurfa.2023.132338

• 37. [IF=9.231] Haijing Li et al."Dynamic changes in postmortem quality of mirror carp (Cyprinus carpio L.): Based on oxidation reaction and mitochondrial function properties."FOOD CHEMISTRY.2023 Nov;425:136426

• 36. [IF=5.714] Qianqian Liu et al."Enhancement of the immune response via the facilitation of dendritic cell maturation by CD-205 Receptor-mediated Long-circling liposomes acting as an antigen and astragalus polysaccharide delivery system."INTERNATIONAL IMMUNOPHARMACOLO

• 35. [4.855] Jincheng Zhang et al."Water-Soluble Conjugated Polyelectrolytes for Adenosine Triphosphate (ATP) Detection."ACS Applied Polymer Materials.2023;XXXX(XXX):XXX-XXX

• 34. [18.027] Xiaoyu Du et al."Single-Molecule Interconversion between Chiral Configurations of Boronate Esters Observed in a Nanoreactor."ACS Nano.2023;XXXX(XXX):XXX-XXX

• 33. [18.027] Yuqin Wang et al."Discrimination of Ribonucleoside Mono-, Di-, and Triphosphates Using an Engineered Nanopore."ACS Nano.2022;XXXX(XXX):XXX-XXX

• 32. [IF=9.231] Liyuan Ma et al."Fluorescence and colorimetric dual-mode immunoassay based on G-quadruplex/N-methylmesoporphyrin IX and p-nitrophenol for detection of zearalenone."FOOD CHEMISTRY.2023 Feb;401:134190

• 31. [IF=9.083] Jiangwei Xiao et al."Development of an in vitro insulin resistance dissociated model of hepatic steatosis by co-culture system."BioScience Trends

• 30. [IF=3.882] Shixuan Sun et al."Amino Acid-Mediated Synthesis of the ZIF-8 Nanozyme That Reproduces Both the Zinc-Coordinated Active Center and Hydrophobic Pocket of Natural Carbonic Anhydrase."Langmuir. 2022;38(4):1621–1630

• 29. [IF=4.155] Yucui Jiang et al."Zr-based acid-stable nucleotide coordination polymers: An excellent platform for acidophilic enzymes immobilization."J Inorg Biochem. 2021 Mar;216:111338

• 28. [IF=5.084] Maochao Zheng et al."Poly(l-ornithine)-Grafted Zinc Phthalocyanines as Dual-Functional Antimicrobial Agents with Intrinsic Membrane Damage and Photothermal Ablation Capacity."Acs Infect Dis. 2021;7(10):2917–2929

• 27. [IF=5.275] Liang Yan et al."New insight into enzymatic hydrolysis of peptides with site-specific amino acid d-isomerization."Bioorg Chem. 2020 Dec;105:104389

• 26. [IF=5.396] Han Liu et al."Astaxanthin attenuates D-galactose-induced brain aging in rats by ameliorating oxidative stress, mitochondrial dysfunction, and regulating metabolic markers."Food Funct. 2020 May;11(5):4103-4113

• 25. [IF=6.986] Jiao Cao et al."A Single-Molecule Observation of Dichloroaurate(I) Binding to an Engineered Mycobacterium smegmatis porin A (MspA) Nanopore."Anal Chem. 2021;93(3):1529–1536

• 24. [IF=7.711] Jinyue Zhang et al."Mapping Potential Engineering Sites of Mycobacterium smegmatis porin A (MspA) to Form a Nanoreactor."Acs Sensors. 2021;6(6):2449–2456

• 23. [IF=7.711] Fubo Ma et al."Nanopore Sequencing Accurately Identifies the Cisplatin Adduct on DNA."Acs Sensors. 2021;6(8):3082–3092

• 22. [IF=14.919] Wang Yuqin et al."Structural-profiling of low molecular weight RNAs by nanopore trapping/translocation using Mycobacterium smegmatis porin A."Nat Commun. 2021 Jun;12(1):1-14

• 21. [IF=4.223] Wang Ruihua et al."UCP2- and non-UCP2-mediated electric current in eukaryotic cells exhibits different properties."Environ Sci Pollut R. 2015 Dec;22(24):19618-19631

• 20. [IF=9.229] Yuqin Wang et al."Osmosis-Driven Motion-Type Modulation of Biological Nanopores for Parallel Optical Nucleic Acid Sensing."Acs Appl Mater Inter. 2018;10(9):7788–7797

• 19. [IF=13.273] Aitang Zhang et al."An efficient and self-guided chemo-photothermal drug loading system based on copolymer and transferrin decorated MoS2 nanodots for dually controlled drug release."Chem Eng J. 2018 Jun;342:120

• 18. Zhang, Li‐Ping, et al. "Effects of 1‐methylcyclopropene on the Metabolic Pathways of Aroma‐Related Compounds in N anguo Pear." Journal of Food Processing and Preservation 38.4 (2014): 1749-1758.https://doi.org/10.1111/jfpp.12138

• 17. Liu, Han, et al. "Astaxanthin attenuates d-galactose-induced brain aging in rats by ameliorating oxidative stress, mitochondrial dysfunction, and regulating metabolic markers." Food & function 11.5 (2020): 4103-4113.https://doi.org/10.1039/D0FO00633E

• 16. Zhang, Aitang, et al. "A Target‐Directed Chemo‐Photothermal System Based on Transferrin and Copolymer‐Modified MoS2 Nanoplates with pH‐Activated Drug Release." Chemistry–A European Journal 23.47 (2017): 11346-11356.https://doi.org/10.1002/chem.201701916

• 15. Aitang Zhang, Aihua Li, Wei Zhao, Guowen Yan, Bingping Liu, Mengli Liu, Meixiu Li, Bingbing Huo, Jingquan Liu, An efficient and self-guided chemo-photothermal drug loading system based on copolymer and transferrin decorated MoS2 nanodots for dually control

• 14. Aitang Zhang, Aihua Li, Wei Zhao, Guowen Yan, Bingping Liu, Mengli Liu, Meixiu Li, Bingbing Huo, Jingquan Liu, An efficient and self-guided chemo-photothermal drug loading system based on copolymer and transferrin decorated MoS2 nanodots for dually control

• 13. Cai, Guangsheng, et al. "Antitumor effect of a liposome-encapsulated β1, 4-galactosyltransferase inhibitor." International journal of pharmaceutics 552.1-2 (2018): 388-393.https://doi.org/10.1016/j.ijpharm.2018.10.010

• 12. Yuqin Wang, Shuanghong Yan, Panke Zhang, Zidao Zeng, Di Zhao, Jiaxin Wang, Hongyuan Chen, and Shuo Huang，Osmosis-Driven Motion-Type Modulation of Biological Nanopores for Parallel Optical Nucleic Acid Sensing，ACS Applied Materials & Interfaces 2018 10 (9),

• 11. Liang Yan, Yongqi Ke, Yuhe Kan, Dao Lin, Jingkui Yang, Yujian He, Li Wu, New insight into enzymatic hydrolysis of peptides with site-specific amino acid d-isomerization, Bioorganic Chemistry, Volume 105, 2020, 104389, ISSN 0045-2068, https://doi.org/10.101

• 10. Wang, Ruihua et al. “A Mechanism for the Temporal Potentiation of Genipin to the Cytotoxicity of Cisplatin in Colon Cancer Cells.” International journal of medical sciences vol. 13,7 507-16. 29 Jun. 2016, doi:10.7150/ijms.15449

• 9. Wang, Ruihua et al. “A Mechanism for the Temporal Potentiation of Genipin to the Cytotoxicity of Cisplatin in Colon Cancer Cells.” International journal of medical sciences vol. 13,7 507-16. 29 Jun. 2016, doi:10.7150/ijms.15449

• 8. 馬伏寧,黃東梅,宋順,吳斌,郭剛,王達(dá)新,譚琳.山地五月茶提取物對(duì)乳腺癌細(xì)胞MDA-MB-231增殖的抑制活性研究[J].熱帶作物學(xué)報(bào),2020,41(08):1693-1699.

• 7. 紀(jì)淑娟,董玲,周鑫,李蕊,程順昌.水楊酸對(duì)1-MCP處理南果梨冷藏后酯類香氣的影響及作用機(jī)理[J].中國(guó)食品學(xué)報(bào),2016,16(06):167-173.

• 6. 張麗萍, 紀(jì)淑娟. 冷藏后不同處理對(duì)1-MCP處理南果梨酯類物質(zhì)及乙烯合成關(guān)鍵酶的影響[J]. 食品科學(xué), 2013(24):285-288.

• 5. 霍仕霞,彭曉明,高莉,黃毅,甘萍,閆明.高良姜素對(duì)過(guò)氧化氫誘導(dǎo)的A375細(xì)胞氧化損傷后Nrf2、γ-GCS基因表達(dá)的影響[J].中國(guó)中醫(yī)藥信息雜志,2015,22(11):69-72.

• 4. 彭曉明, 霍仕霞, 高莉, et al. 高良姜素對(duì)H_2O_2誘導(dǎo)A375細(xì)胞氧化損傷的保護(hù)作用[J]. 西北藥學(xué)雜志, 2015(1):51-54.

• 3. 賈葉葉, 田洪磊, 詹萍,等. 酶法制備沙漠果蛋白ACE抑制肽工藝的研究[J]. 食品工業(yè)科技, 2016, 37(06):264-269.

• 2. 李棟, 楊淑燕, 郭峰. 水潤(rùn)滑環(huán)境下聚合物PLL-g-PEG的宏觀摩擦學(xué)性能的研究[J]. 摩擦學(xué)學(xué)報(bào), 2019, 039(004):434-443.

• 1. 呂遠(yuǎn)奇 詹萍 李江 等. 響應(yīng)面法優(yōu)化新疆馬鹿鹿茸蛋白質(zhì)酶解工藝[J]. 食品工業(yè) 2017(6):75-79.

輸入產(chǎn)品批號(hào)：

本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *