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• 產(chǎn)品描述： WS3, a β cell proliferation inducer, regulates Erb3 binding protein-1 (EBP1) and the IκB kinase pathway

• 靶點(diǎn)： EGFR;IκB/IKK

• 體外研究：
  WS3 induces pancreatic R7T1 β cell proliferation in dose response, with an EC50 value of 0.28 μM.
  WS3 (1.0 nM-1.0 μM) reversibly proliferate primary retinal pigment epithelial (RPE) cells isolated from fetal and adult human donors. Following withdrawal of WS3, RPE cells differentiate into a functional monolayer, as exhibited by their expression of mature RPE genes and phagocytosis of photoreceptor outer segments.

• 體內(nèi)研究：
  在小鼠β細(xì)胞系R7T1中,WS3誘導(dǎo)細(xì)胞增殖（EC50=0.074 μM）。在原代胚胎和成人視網(wǎng)膜色素上皮細(xì)胞中,WS3可逆誘導(dǎo)細(xì)胞發(fā)生增殖

• 細(xì)胞實(shí)驗(yàn)： R7T1 cells are growth-arrested by removal of doxycycline for 2 days and plated into 384-well plates at a density of 3,000 cells/well in growth medium. Β cell proliferation is assessed using CellTiter Glo after 4 days. Fold increase in cell number is calculated by normalizing compound-treated wells to the median of DMSO-treated wells. (Only for Reference)

• 參考文獻(xiàn)：
  1. Shen W, et al. Small-molecule inducer of β cell proliferation identified by high-throughput screening. J Am Chem Soc. 2013 Feb 6;135(5):1669-72. 2. Swoboda JG, et al. Small molecule mediated proliferation of primary retinal pigment epithelial cells. ACS Chem Biol. 2013, 8(7), 1407-1411.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存條件： -20℃

• 配置溶液濃度參考：
  

  	1mg	5mg	10mg
  1 mM	1.756 ml	8.778 ml	17.557 ml
  5 mM	0.351 ml	1.756 ml	3.511 ml
  10 mM	0.176 ml	0.878 ml	1.756 ml
  50 mM	0.035 ml	0.176 ml	0.351 ml

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶(hù)參考交流研究之用。

輸入產(chǎn)品批號(hào)：

本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *