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• 產(chǎn)品描述： EMD638683 是一種 serum- and glucocorticoid-inducible kinase 1 (SGK1) 的高度選擇性抑制劑，其IC50值為3 μM。EMD638683 具有降壓藥效和抗腫瘤活性

• 靶點(diǎn)： SGK1(Cell-free assay):3 μM;SGK

• 體內(nèi)研究：
  The in vivo development of tumors following chemical carcinogenesis is significantly blunted by treatment with EMD638683. Within 24 hours in vivo EMD638683 treatment significantly decreases blood pressure in fructose/saline-treated mice but not in control animals or in SGK1 knockout mice

• 細(xì)胞實(shí)驗(yàn)： Cell lines: HeLa-cells Concentrations: 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM Incubation Time: 24 h Method: The cells are plated in 6-wells MTPs at a density of 10–20 × 103 cells/cm2 in Dulbecco′s DMEM medium, supplemented with 10% fetal calf serum (FCS), 2 mM glutamine and 1 mM sodium pyruvate. After 24 hrs at 37℃ and 5% CO2 in a cell incubator, each well is further supplemented with 25 μl of a 100X dimethyl sulfoxide (DMSO) solution of the compound; the solution is diluted 100-fold in the supernatant of the cell culture, thus resulting in the anticipated SGK1-inhibitor concentration at a 1% DMSO concentration.

• 動(dòng)物實(shí)驗(yàn)： Animal Models: 8-week-old wild type mice Dosages: 20 mg/kg, 600 mg/kg Administration: IP, Oral gavage

• 參考文獻(xiàn)：
  1. Teresa F Ackermann, et al. Cell Physiol Biochem. 2011;28(1):137-46. 2. Syeda T Towhid, et al. Cell Physiol Biochem. 2013;32(4):838-48.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存條件： -20℃

• 配置溶液濃度參考：
  

  	1mg	5mg	10mg
  1 mM	2.745 ml	13.723 ml	27.447 ml
  5 mM	0.549 ml	2.745 ml	5.489 ml
  10 mM	0.274 ml	1.372 ml	2.745 ml
  50 mM	0.055 ml	0.274 ml	0.549 ml

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

輸入產(chǎn)品批號(hào)：

本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *