• 產(chǎn)品描述：

  GC376 是一種 3CLpro (3C-like protease) 的抑制劑，對于PEDV 3CLpro的IC50值約為1.11 μM。GC376 對多種冠狀病毒（包括SARS-CoV）的3CLpro有活性

• 靶點(diǎn)： PEDV 3CLpro(Cell-free assay):1.11 μM;SARS-CoV

• 體外研究：
  Molnupiravir and GC376 show a synergistic activity on SARS-CoV-2 at 48 h, and an additive activity on SARS-CoV-2 at 72 h

• 體內(nèi)研究：
  GC376, a dipeptidyl bisulfite adduct salt, is a 3CLpro (3C-like protease) inhibitor that exerts strong inhibitory effects on picornaviruses and coronaviruses, including SARS-CoV.

• 細(xì)胞實(shí)驗(yàn)： Cell lines: Vero E6 cells Concentrations: 0.21-16.7 μM Incubation Time: 48 and 72 h Method: Vero E6 cells (3000 cells/well) were seeded in 96-well clear flat-bottom plates and incubated at 37°C with 5% CO2 for 24 h. After incubation, cells were infected using a multiplicity of infection (MOI) of 0.1. SARS-CoV-2 was allowed to adsorb for one hour at 37°C. Subsequently, virus inoculum was removed, and cells were overlaid with media containing 3-fold serial dilutions of molnupiravir (0.62-50 μM), nirmatrelvir (0.62-50 μM) and GC376 (0.21-16.7 μM). Negative controls (compounds alone), infected positive controls (SARS-CoV-2 alone) and mock-infected cells were included in each plate. Plates were incubated at 37°C with 5% CO2 for 48 and 72 h and then, cell viability was measured using an MTT reduction assay.

• 動物實(shí)驗(yàn)： Animal Models: Female BALB/c mice Dosages: 111 or 55.5 mg/kg Administration: i.m.

• 參考文獻(xiàn)：
  1. Gang Ye, et al. Structural Basis for Inhibiting Porcine Epidemic Diarrhea Virus Replication with the 3C-Like Protease Inhibitor GC376. Viruses. 2020 Feb 21;12(2):240. 2. Gidari A, et al. The Combination of Molnupiravir with Nirmatrelvir or GC376 Has a Synergic Role in the Inhibition of SARS-CoV-2 Replication In Vitro. Microorganisms. 2022 Jul 21;10(7):1475. 3. Shi Y, et al. The preclinical inhibitor GS441524 in combination with GC376 efficaciously inhibited the proliferation of SARS-CoV-2 in the mouse respiratory tract. Emerg Microbes Infect. 2021 Dec;10(1):481

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存條件： -20℃，充氮保存

• 配置溶液濃度參考：
  

  	1mg	5mg	10mg
  1 mM	1.97 ml	9.852 ml	19.703 ml
  5 mM	0.394 ml	1.97 ml	3.941 ml
  10 mM	0.197 ml	0.985 ml	1.97 ml
  50 mM	0.039 ml	0.197 ml	0.394 ml

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

• 1. [IF=14.903] Yang Yi et al."Schaftoside inhibits 3CLpro and PLpro of SARS-CoV-2 virus and regulates immune response and inflammation of host cells for the treatment of COVID-19."Acta Pharmaceutica Sinica B.2022 Aug;:

輸入產(chǎn)品批號：

本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *