• 介紹：
  溴化十六烷基三甲胺：CTAB是一種陽離子去污劑，具有從低離子強(qiáng)度的溶液中沉淀核酸和酸性多聚糖的特性，在這種條件下，蛋白質(zhì)和中性多聚糖仍留在溶液里，在高離子強(qiáng)度的溶液里，與蛋白質(zhì)和大多數(shù)酸性多聚糖以外的多聚糖形成復(fù)合物，只是不能沉淀核酸。因此，CTAB可以用于從大量產(chǎn)生粘多糖的有機(jī)體如植物以及某些革蘭氏陰性菌(包括E.coli的某些株)中制備純化DNA 。

  CTAB原理：CTAB是一種陽離子去污劑，具有從低離子強(qiáng)度的溶液中沉淀核酸和酸性多聚糖的特性，在這種條件下，蛋白質(zhì)和中性多聚糖仍留在溶液里，在高離子強(qiáng)度的溶液里，能夠與蛋白質(zhì)和大多數(shù)酸性多聚糖以外的多聚糖形成復(fù)合物，只是不能沉淀核酸，從而將核酸分離出來。

  CTAB使用方法：

  1.提取DNA配方：

  CTAB 4g

  NaCl 16.364 g

  1M Tris-HCl 20 mL( PH8.0)

  冷卻后0.2-1%2-巰基乙醇（400ul）氯仿-異戊醇（24：1）：先加96ml氯仿，再加4ml異戊醇，搖勻即可。

  2.提取RNA配方

  2% CTAB（W/V）

  2%聚乙烯吡咯烷酮PVP（W/V）

  100 mM Tris-HCl（pH8.0,DEPC處理的水配制）

  25mM EDTA

  由于在高溫滅菌條件下，Tris-HCl要和DEPC發(fā)生反應(yīng)，所以配RNA提取緩沖液時(shí)直接用DEPC 處理的水

  CTAB使用注意事項(xiàng)：具有刺激性，若吸入、攝入或皮膚吸收，會對人體有害。使用時(shí)要戴合適的手套和防護(hù)眼鏡（或在通風(fēng)櫥操作）。避免吸入其粉塵。

• 熔點(diǎn)： 248-251℃

• 外觀： 白色結(jié)晶

• 溶解性： Souble  in  EtOH

• 敏感性： 易吸潮

• 儲存條件： RT

• 用途： 催化劑,乳化劑,消毒劑,殺菌基,抗靜電劑等。比色測定或光度測定銻和錫。腸內(nèi)葡萄糖吸收抑制劑。芳烴還原和烯環(huán)氧化時(shí)的相轉(zhuǎn)移催化劑。

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

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輸入產(chǎn)品批號：

本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *