十六烷基三甲基溴化銨

    AR,99%

CTAB

源葉
S15001
57-09-0
C19H42BrN
364.45
MFCD00011772
鯨蠟烷三甲基溴化銨;西曲溴銨;溴棕三甲銨
品牌 貨號 產(chǎn)品規(guī)格 價(jià)格(RMB) 庫存(上海) 北京 武漢 南京 購買數(shù)量
源葉 S15001-100g AR,99% ¥60.00元 >10 4 - 2
源葉 S15001-500g AR,99% ¥198.00元 >10 1 2 -
源葉 S15001-5*500g AR,99% ¥900.00元 10 - - -
產(chǎn)品介紹 參考文獻(xiàn)(46篇) 質(zhì)檢證書(COA) 摩爾濃度計(jì)算器 相關(guān)產(chǎn)品

產(chǎn)品介紹

溴化十六烷基三甲胺:CTAB是一種陽離子去污劑,具有從低離子強(qiáng)度的溶液中沉淀核酸和酸性多聚糖的特性,在這種條件下,蛋白質(zhì)和中性多聚糖仍留在溶液里,在高離子強(qiáng)度的溶液里,與蛋白質(zhì)和大多數(shù)酸性多聚糖以外的多聚糖形成復(fù)合物,只是不能沉淀核酸。因此,CTAB可以用于從大量產(chǎn)生粘多糖的有機(jī)體如植物以及某些革蘭氏陰性菌(包括E.coli的某些株)中制備純化DNA 。
CTAB原理:CTAB是一種陽離子去污劑,具有從低離子強(qiáng)度的溶液中沉淀核酸和酸性多聚糖的特性,在這種條件下,蛋白質(zhì)和中性多聚糖仍留在溶液里,在高離子強(qiáng)度的溶液里,能夠與蛋白質(zhì)和大多數(shù)酸性多聚糖以外的多聚糖形成復(fù)合物,只是不能沉淀核酸,從而將核酸分離出來。
CTAB使用方法:
1.提取DNA配方:
CTAB 4g
NaCl 16.364 g
1M Tris-HCl 20 mL( PH8.0)
冷卻后0.2-1%2-巰基乙醇(400ul)氯仿-異戊醇(24:1):先加96ml氯仿,再加4ml異戊醇,搖勻即可。
2.提取RNA配方
2% CTAB(W/V)
2%聚乙烯吡咯烷酮PVP(W/V)
100 mM Tris-HCl(pH8.0,DEPC處理的水配制)
25mM EDTA
由于在高溫滅菌條件下,Tris-HCl要和DEPC發(fā)生反應(yīng),所以配RNA提取緩沖液時(shí)直接用DEPC 處理的水
CTAB使用注意事項(xiàng):具有刺激性,若吸入、攝入或皮膚吸收,會對人體有害。使用時(shí)要戴合適的手套和防護(hù)眼鏡(或在通風(fēng)櫥操作)。避免吸入其粉塵。
熔點(diǎn): 248-251℃
外觀: 白色結(jié)晶
溶解性: Souble  in  EtOH
敏感性: 易吸潮
儲存條件: RT
用途: 催化劑,乳化劑,消毒劑,殺菌基,抗靜電劑等。比色測定或光度測定銻和錫。腸內(nèi)葡萄糖吸收抑制劑。芳烴還原和烯環(huán)氧化時(shí)的相轉(zhuǎn)移催化劑。
注意: 部分產(chǎn)品我司僅能提供部分信息,我司不保證所提供信息的權(quán)威性,僅供客戶參考交流研究之用。

參考文獻(xiàn)(46篇)

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