羅丹明123

    生物技術(shù)級,98%(HPLC)

Rhodamine 123

源葉
S19123
62669-70-9
C21H17ClN2O3
380.82
MFCD00012664
品牌 貨號 產(chǎn)品規(guī)格 價格(RMB) 庫存(上海) 北京 武漢 南京 購買數(shù)量
源葉 S19123-5mg 生物技術(shù)級,98%(HPLC) ¥140.00元 >10 - 2 4
源葉 S19123-25mg 生物技術(shù)級,98%(HPLC) ¥290.00元 3 - 3 -
源葉 S19123-100mg 生物技術(shù)級,98%(HPLC) ¥890.00元 2 - - -
源葉 S19123-500mg 生物技術(shù)級,98%(HPLC) ¥3200.00元 預(yù)計交期:2-3天 - - -
源葉 S19123-1g 生物技術(shù)級,98%(HPLC) ¥5600.00元 預(yù)計交期:2-3天 - - -
產(chǎn)品介紹 參考文獻(xiàn)(27篇) 質(zhì)檢證書(COA) 摩爾濃度計算器 相關(guān)產(chǎn)品

產(chǎn)品介紹

羅丹明123 (Rhodamine 123、羅丹明123, Rh 123)是一種常用的陽離子綠色熒光染料,可以透過細(xì)胞膜從而在活細(xì)胞線粒體內(nèi)聚集,并發(fā)出黃綠色熒光。廣泛用作檢測線粒體膜電位的熒光探針,并且對細(xì)胞沒有任何毒性。 

溶液的配制

用DMSO溶解,終濃度為1-5 mM。溶液分裝,避光保存于-20 ºC。

羅丹明123使用方法

1. 用載玻片準(zhǔn)備細(xì)胞。細(xì)胞數(shù)目應(yīng)為 5×104~5×105個/mL。

2. 孵育該載玻片,用 PBS或Hank’s 液洗滌細(xì)胞。

3. 用培養(yǎng)基稀釋 Rh123母液以制備 1~20µM Rh123緩沖液。具體工作濃度取決于細(xì)胞類型和細(xì)胞濃度。

4. 將 Rh123緩沖液加入載玻片并在37℃下孵育 30 分鐘至 1 小時。

5. 去除 Rh123 緩沖液并用培養(yǎng)基洗滌細(xì)胞(洗滌細(xì)胞后為了固定,加入 10%福爾馬林緩沖液并孵育 15~20 分鐘,接著用 PBS洗滌)。

6. 用帶有熒光素濾光片的熒光顯微鏡觀察細(xì)胞。

為了您的安全和健康,請穿實驗服并戴一次性手套操作。

熔點: 235℃
溶解性: Soluble  in  ethanol  (20  mg/ml),  DMSO,  methanol,  and  water  (partly  miscible).
儲存條件: RT
注意: 部分產(chǎn)品我司僅能提供部分信息,我司不保證所提供信息的權(quán)威性,僅供客戶參考交流研究之用。

參考文獻(xiàn)(27篇)

22. [IF=3.2] Ziqin Wei et al."Regulatory mechanism of ABCB1 transcriptional repression by HDAC5 in rat hepatocytes under hypoxic environment."Frontiers in Physiology.2025 Apr;16:1520246 21. [IF=8.2] Zhen Cheng et al."Construction of nanoparticles from blueberry anthocyanins-lecithin/gum Arabic improves lipid droplet accumulation and gut microbiota disturbance in HFD-induced obese mice."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES".2024 Apr;264: 20. [IF=2.7] Haoying Li et al."Mechanistic investigation of the ameliorative effect of liquiritin on hypoxia/reoxygenation?induced cardiomyocyte injury based on network pharmacology and in?vitro validation."Experimental and Therapeutic Medicine.2024 Mar;27(3):1-15 19. [IF=5.6] Jiaying Qi et al."Mechanistic insights into the ameliorative effects of hypoxia-induced myocardial injury by Corydalis yanhusuo total alkaloids: based on network pharmacology and experiment verification."Frontiers in Pharmacology.2023; 14: 1275558 18. [IF=5.318] Xiaoqiang Chen et al."Green tea polysaccharide conjugates and gelatin enhanced viability of L. acidophilus by layer-by-layer encapsulation."Food Bioscience.2023 Feb;:102471 17. [IF=5.222] Mei Li et al."Potential Pharmacokinetic Effect of Chicken Xenobiotic Receptor Activator on Sulfadiazine: Involvement of P-glycoprotein Induction."Antibiotics-Basel.2022 Aug;11(8):1005 16. [IF=5.911] Zhiling Tang et al."Unraveling the antibacterial mechanism of 3-carene against Pseudomonas fragi by integrated proteomics and metabolomics analyses and its application in pork."INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY.2022 Oct;379:109846 15. [IF=6.388] Muqing Zhang et al."Liquiritigenin protects against myocardial ischemic by inhibiting oxidative stress, apoptosis, and L-type Ca2+ channels."PHYTOTHERAPY RESEARCH 14. [IF=2.863] Bin Zheng et al."10-Gingerol alleviates hypoxia/reoxygenation-induced cardiomyocyte injury through inhibition of the Wnt5a/Frizzled-2 pathway."Food Sci Nutr. 2021 Jul;9(7):3917-3931 13. [IF=3.738] Xue Liu et al."Antibacterial activity and mechanism of linalool against Pseudomonas aeruginosa."Microb Pathogenesis. 2020 Apr;141:103980 12. [IF=4.411] Fengyu Guo et al."Antibacterial Activity and Mechanism of Linalool against Shewanella putrefaciens."Molecules. 2021 Jan;26(1):245 11. [IF=4.411] Huizhen Shu et al."Antimicrobial Activity and Proposed Action Mechanism of 3-Carene against Brochothrix thermosphacta and Pseudomonas fluorescens."Molecules. 2019 Jan;24(18):3246 10. [IF=5.396] Han Liu et al."Astaxanthin attenuates D-galactose-induced brain aging in rats by ameliorating oxidative stress, mitochondrial dysfunction, and regulating metabolic markers."Food Funct. 2020 May;11(5):4103-4113 9. [IF=5.64] Fengyu Guo et al."Antimicrobial Activity and Proposed Action Mechanism of Linalool Against Pseudomonas fluorescens."Front Microbiol. 2021 Jan 28;12:562094 8. [IF=6.419] Junqiu Zhai et al."Exenatide-loaded inside-porous poly(lactic-co-glycolic acid) microspheres as a long-acting drug delivery system with improved release characteristics."Drug Deliv. 2020;27(1):1667-1675 7. Zhai, Junqiu, et al. "Exenatide-loaded inside-porous poly (lactic-co-glycolic acid) microspheres as a long-acting drug delivery system with improved release characteristics." Drug Delivery 27.1 (2020): 1667-1675.https://doi.org/10.1080/10717544.2020.185091 6. Liu, Han, et al. "Astaxanthin attenuates d-galactose-induced brain aging in rats by ameliorating oxidative stress, mitochondrial dysfunction, and regulating metabolic markers." Food & function 11.5 (2020): 4103-4113.https://doi.org/10.1039/D0FO00633E 5. 郭俸鈺, 陳文學(xué), 陳海明,等. 芳樟醇對大腸桿菌的抑菌作用機制[J]. 現(xiàn)代食品科技, 2020(4):113-118. 4. 郁莉, 于振蘭, 賈艷麗. 舒尼替尼對吉非替尼耐藥的肺癌細(xì)胞的治療效果及意義[J]. 中國藥學(xué)雜志, 2017(03):244-248. 3. 劉雪, 王靜楠, 陳文學(xué),等. 檸檬烯對銅綠假單胞菌的抑菌活性及其機理[J]. 食品工業(yè)科技, 2018, 039(007):1-5. 2. 舒慧珍, 唐志凌, 劉雪,等. 檸檬烯對熒光假單胞菌抑菌活性及機理研究[J]. 食品工業(yè)科技, 2019, v.40;No.428(12):140-146. 1. 劉雪 何英蘭 胡月英 等. 3-蒈烯對銅綠假單胞菌的抑菌活性及機理初探[J]. 熱帶作物學(xué)報 2019 40(03):191-198.

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摩爾濃度計算器

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