| 產(chǎn)品描述： | SBE13 Hydrochloride is a potent and selective Plk1 inhibitor, with an IC50 of 200 pM; SBE13 Hydrochloride poorly inhibits Plk2 (IC50>66?μM) or Plk3 (IC50=875?nM). |
| 靶點(diǎn)： |
PLK1:200 pM (IC50);PLK3:875 nM (IC50);Apoptosis;?PLK;?Autophagy |
| 體外研究： |
SBE13 significantly reduce cell proliferation and induce apoptosis in HeLa cells, with an EC50 of 18?μM. SBE13 (1-100 μM) shows no effect on Caspase 3/7 activity in NIH-3T3 cells. SBE13 (66 and 100 μM) does not change morphology after treatment of primary cells. SBE13 (10 and 100 μM) reduces pRb staining in primary cells, and this indicates a G0/G1 arrest. SBE13 (66 and 100 μM) increases levels of cyclin B1, phospho histone H3, Wee1, Emi1 and securin, and results in cleavage of Cdc27 in HeLa cells. SBE13 (10 and 100 μM) also induces apoptosis of HeLa cells |
| 參考文獻(xiàn)： |
1. Keppner S, et al. Identification and validation of a potent type II inhibitor of inactive polo-like kinase 1. ChemMedChem. 2009 Nov;4(11):1806-9. 2. Keppner S, et al. Fate of primary cells at the G?/S boundary after polo-like kinase 1 inhibition by SBE13. Cell Cycle. 2011 Feb 15;10(4):708-20. Epub 2011 Feb 15. 3. Keppner S, et al. Biological impact of freezing Plk1 in its inactive conformation in cancer cells. Cell Cycle. 2010 Feb 15;9(4):761-73. Epub 2010 Feb 16. |
| 溶解性： |
Soluble in DMSO、H2O |
| 保存條件： |
-20℃ |
| 配置溶液濃度參考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.086 ml |
10.43 ml |
20.86 ml |
| 5 mM |
0.417 ml |
2.086 ml |
4.172 ml |
| 10 mM |
0.209 ml |
1.043 ml |
2.086 ml |
| 50 mM |
0.042 ml |
0.209 ml |
0.417 ml |
|
| 注意： |
部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。 |
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營業(yè)執(zhí)照(三證合一)
北京