| 產(chǎn)品描述： | Lucanthone is an inhibitor of Apurinic endonuclease-1 (APE-1). |
| 靶點(diǎn)： |
Autophagy;DNA/RNA Synthesis;DNA/RNASynthesis;Autophagy |
| 細(xì)胞實(shí)驗(yàn)： |
Cell viability is assessed by MTT assay. Cells are seeded into 96-well microculture plates at 10,000 cells per well and allowed to attach for 24 h. Cells are then treated with Lucanthone (0, 0.5, 1, 5, 10, 20 and 40 μM), Chloroquine, Vorinostat, or combinations for 72 h. Following drug treatment, MTT is added and cell viability is quantified using a BioTek microplate reader. Effects on cell viability are also determined by measuring ATP levels using the ATPlite assay system and by trypan blue exclusion. Pro-apoptotic effects following in vitro drug exposure are quantified by propidium iodide (PI) staining and fluorescence-activated cell sorting (FACS) analysis of sub-G0/G1 DNA content |
| 參考文獻(xiàn)： |
1. Carew JS,etal.Lucanthone is a novel inhibitor of autophagy that induces cathepsin D-mediated apoptosis.J Biol Chem. 2011 Feb 25;286(8):6602-13. 2. Chowdhury SM,etal.Graphene nanoribbons as a drug delivery agent for lucanthone mediated therapy of glioblastoma multiforme.Nanomedicine. 2015 Jan;11(1):109-18. |
| 溶解性： |
Soluble in DMSO |
| 保存條件： |
2-8℃ |
| 配置溶液濃度參考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.937 ml |
14.685 ml |
29.37 ml |
| 5 mM |
0.587 ml |
2.937 ml |
5.874 ml |
| 10 mM |
0.294 ml |
1.469 ml |
2.937 ml |
| 50 mM |
0.059 ml |
0.294 ml |
0.587 ml |
|
| 注意： |
部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。 |
上海工商
危險(xiǎn)品化學(xué)品經(jīng)營許可證（不帶存儲(chǔ)）
營業(yè)執(zhí)照(三證合一)
北京